Medizinisches Proteom-Center
Medical Proteome Analysis, Center for Proteindiagnostics (PRODI)
Faculty of Medicine
Ruhr University Bochum
Gesundheitscampus 4
44801 Bochum
Room:
E2.259
Phone:
+49 (0)234 32-18106
Email:
katrin.marcus@ruhr-uni-bochum.de
Since 2014, I have been head of the Medizinisches Proteom-Center at the Medical Faculty of the Ruhr-University Bochum. My expertise is in the field of medical proteome analysis with a special focus on neurodegenerative diseases such as Parkinson's disease. Together with many cooperation partners, we are searching for the underlying pathomechanisms of these diseases. In addition, using the latest approaches in proteomics and through continuous development of available technologies, we are searching for protein biomarkers that allow early and progressive diagnosis of these diseases. For these applications, we have developed a comprehensive, statistically validated analysis strategy that includes sophisticated sample preparation for a wide variety of sample material (tissues, body fluids, cells, etc.), protein or peptide separation, detection of modified protein species, protein identification/quantification by mass spectrometry and validation of candidate proteins by antibody-based methods and targeted mass spectrometry. Increasingly, we are now also entering the field of functional proteomics using methods of molecular biology, cell culture, various functional assays and immunohistochemistry with the help of which we are able to shed light on the functions of individual identified proteins. Recently, we have also added lipidomics to our portfolio. Bioinformatics has become a very important part of our work over the years, and we are developing tools and approaches here to optimally analyse our extensive data, integrate OMICS data with clinical data and improve statistics.
Zang, J. C. S., May, C., Hellwig, B., Moser, D., Hengstler, J. G., Cole, S., Heinrichs, M., Rahnenführer, J., Marcus, K., & Kumsta, R. (2023). Proteome analysis of monocytes implicates altered mitochondrial biology in adults reporting adverse childhood experiences. Translational Psychiatry, 13(1), 31. https://doi.org/10.1038/s41398-023-02320-w
Wulf, M., Barkovits, K., Schork, K., Eisenacher, M., Riederer, P., Gerlach, M., Eggers, B., & Marcus, K. (2022). Neuromelanin granules of the substantia nigra: Proteomic profile provides links to tyrosine hydroxylase, stress granules and lysosomes. Journal of Neural Transmission (Vienna, Austria : 1996), 129(10), 1257–1270. https://doi.org/10.1007/s00702-022-02530-4
Caldi Gomes, L., Roser, A.‑E., Jain, G., Pena Centeno, T., Maass, F., Schilde, L., May, C., Schneider, A., Bähr, M., Marcus, K., Fischer, A., & Lingor, P. (2021). MicroRNAs from extracellular vesicles as a signature for Parkinson's disease. Clinical and Translational Medicine, 11(4), e357. https://doi.org/10.1002/ctm2.357
Barkovits, K., Kruse, N., Linden, A., Tönges, L., Pfeiffer, K., Mollenhauer, B., & Marcus, K. (2020). Blood Contamination in CSF and Its Impact on Quantitative Analysis of Alpha-Synuclein. Cells, 9(2). https://doi.org/10.3390/cells9020370
Barkovits, K., Pacharra, S., Pfeiffer, K., Steinbach, S., Eisenacher, M., Marcus, K. & Uszkoreit, J. (2020). Reproducibility, Specificity and Accuracy of Relative Quantification Using Spectral Library-based Data-independent Acquisition. Molecular & Cellular Proteomics, 19(1), 181–197. https://doi.org/10.1074/mcp.ra119.001714
Boussaad, I., Obermaier, C. D., Hanss, Z., Bobbili, D. R., Bolognin, S., Glaab, E., Wołyńska, K., Weisschuh, N., Conti, L. de, May, C., Giesert, F., Grossmann, D., Lambert, A., Kirchen, S., Biryukov, M., Burbulla, L. F., Massart, F., Bohler, J., Cruciani, G., Marcus, K., . . . Krüger, R. (2020). A patient-based model of RNA mis-splicing uncovers treatment targets in Parkinson's disease. Science Translational Medicine, 12(560). https://doi.org/10.1126/scitranslmed.aau3960
Plum, S., Eggers, B., Helling, S., Stepath, M., Theiss, C., Leite, R. E. P., Molina, M., Grinberg, L. T., Riederer, P., Gerlach, M., May, C., & Marcus, K. (2020). Proteomic Characterization of Synaptosomes from Human Substantia Nigra Indicates Altered Mitochondrial Translation in Parkinson's Disease. Cells, 9(12). https://doi.org/10.3390/cells9122580
Kley, R. A., Maerkens, A., Leber, Y., Theis, V., Schreiner, A., van der Ven, P. F. M., Uszkoreit, J., Stephan, C., Eulitz, S., Euler, N., Kirschner, J., Müller, K., Meyer, H. E., Tegenthoff, M., Fürst, D. O., Vorgerd, M., Müller, T., & Marcus, K. (2013). A combined laser microdissection and mass spectrometry approach reveals new disease relevant proteins accumulating in aggregates of filaminopathy patients. Molecular & Cellular Proteomics : MCP, 12(1), 215–227. https://doi.org/10.1074/mcp.M112.023176
Tribl, F., Asan, E., Arzberger, T., Tatschner, T., Langenfeld, E., Meyer, H. E., Bringmann, G., Riederer, P., Gerlach, M., & Marcus, K. (2009). Identification of L-ferritin in neuromelanin granules of the human substantia nigra: A targeted proteomics approach. Molecular & Cellular Proteomics : MCP, 8(8), 1832–1838. https://doi.org/10.1074/mcp.M900006-MCP200
Klose, J., Nock, C., Herrmann, M., Stühler, K., Marcus, K., Blüggel, M., Krause, E., Schalkwyk, L. C., Rastan, S., Brown, S. D. M., Büssow, K., Himmelbauer, H., & Lehrach, H. (2002). Genetic analysis of the mouse brain proteome. Nature Genetics, 30(4), 385–393. https://doi.org/10.1038/ng861
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Last update: Feb 28, 2024
